Perpustakaan Digital ITB

Advances in nanoparticle design have led to thedevelopment of nanoparticulate systems that can sense intra-cellular molecules, alter cellular processes, and release drugs tospecific targets in vitro. In this work, we demonstrate thatoligonucleotide-coated gold nanoparticles are suitable for thedetection of mRNA in liveHydra vulgaris, a model organism,without affecting the animal’s integrity. We specifically focus onthe detection of Hymyc1 mRNA, which is responsible for theregulation of the balance between stem cell self-renewal anddifferentiation. Myc deregulation is found in more than half of human cancers, thus the ability to detect in vivo related mRNAsthrough innovativefluorescent systems is of outmost interest.