Perpustakaan Digital ITB

In terms of detection of antibiotics withincomplex media, the nonspecific adsorption is an enormouschallenge and antifouling sensing interfaces capable of reducingthe nonspecific adsorption from complex biological samples arehighly desirable. In this work, a novel antifouling electrochemicalimmunosensor was explored based on the self-assembly of twokinds of poly(N-isopropylacrylamide) microgels on the surfaceof graphene oxide for sensitive detection of streptomycin (STR).The microgels modified with glycidyl methacrylate (GMA) andzwitterionic liquid 1-propyl-3-vinylimidazole sulfonate(PVIS) were prepared. The microgels with GMA were used bycombining specific recognition of anti-STR. The rapid specificbinding of antigen and anti-STR resulted in a decrease of currentdensity to generate electrochemical responsive signals. Zwitter-ionic liquid-modified microgels were used for antifouling, which can form stronger hydration and show excellent antifoulingability. As a result, we achieved efficient and sensitive detection of STR in the complex sample with evidently resistednonspecific adsorption effect, the wide linear range toward STR was from 0.05 to 100 ng mL?1, with a detection limit down to1.7 pg mL?1. The immunosensor based on the surface functionalization of microgels showed promising applications for thedetection of antibiotics in complex media.