Perpustakaan Digital ITB

Ethical debates about Embryonic Stem Cell focus on obtaining preimplantation embryos, as this sacrifices potential infants. To address this, BGI is studying if more accessible induced pluripotent stem cells can be used in primed-to-preimplantation inducing mediums. This study aims to compare the differences in known preimplantation stage markers in cells undergoing different induction methods. Determining variable genes, differentially expressed genes, and upregulated pathways between 12-Day (4CL medium) H9 embryonic stem cell line and public 12-Day (5iLAF medium) G15.AO induced pluripotent stem cells, analysed differentially expressed genes were dot-plotted to select for pre-implantation stage markers. Followed by induction of Pluripotent primed stem cells of embryonic and induced origin in 4CL, a primed-to-preimplantation inducing medium made by BGI, for 12 days. Samples tested for presence of Early Embryo Stage and Preimplantation markers (KLF17, ZFP42, TFCP2L1, DNMT3L) using Quantitative Polymerase Chain Reaction. Shared variable gene S100A6’s, determined to impact the efficiency of post-to-preimplantation induction. via CacyBP/SIP inhibition, acting as WNT inhibitor, through degradation of b-catenin. Independent T-tests of QPCR showed, H9 and U2 samples, cell lines had no significant difference collectively, and primed Day 1 and 4CL Day 12 samples did. Before 4CL induction. U2 is already producing REX1 at a higher expression, further induced by 4CL medium. TFCP2L1 and DNMT3L results show U2 is more conducive for epiblast-like cells while H9 favours blastocysts. REX1 shows remnant signs of WNT signal alterations from its previous induction process, adding variability in using pre-induced cell lines for post-to-preimplantation induction.