Perpustakaan Digital ITB

Centella asiatica (CA) is known as herbal medicine with a number therapeutical effects to many diseases and abnormalities. It is famous for its effect in neuroprotective and wound-healing properties. As to its effective ability to heal wounds, we want to evaluate the activity of several important constituents in C. asiatica in the other effects on skin, especially skin-aging. Asiaticoside, madecassoside, asiatic acid, and madecassic acid are four of the most abundant constituents in C. asiatica. The presence of the four constituents in the C. asiatica extracts were confirmed using TLC analysis: in ethyl acetate-methanol-water (8:1.5:0.5) eluent for the detection of asiaticoside and madecassoside and chloroform-methanol (9:1) eluent for the detection of asiatic acid and madecassic acid. The anti-aging activity of the four constituents was evaluated within the inhibition activity toward elastase and tyrosinase enzyme in their elastase and tyrosinase inhibition assay. The result showed that the asiaticoside, madecassoside, asiatic acid, and madecassic acid did not possess any anti-elastase activity but a potential activity on inhibiting tyrosinase enzyme. However, a significant and potential activity of tyrosinase inhibition was obtained in the sample of C. asiatica ethyl acetate extract with value above 45 percent with the leading potential compound, madecassic acid.