2020 EJRNL PP Ying Zhang - 1.pdf
Terbatas Irwan Sofiyan
» ITB
Terbatas Irwan Sofiyan
» ITB
Targeted delivery of photosensitizer for antitumor photodynamic
therapy has received much attention recently. In this research, a multitargeting
graphene oxide (GO) nanosystem (GFFP-TPPa) was fabricated based on the
magnetically targeted nano-Fe3O4, active targeted folic acid (FA), and
mitochondria-targeted triphenylphosphine, aiming to provide an effective drug
delivery system of photosensitizer for improved photodynamic therapy against
human tumor. The effective photosensitizer release from GFFP-TPPa under
mild acidic pH condition (such as tumor microenvironment) suggested our
designed nanosystem could reduce cutaneous phototoxicity of nanoparticles
(NPs) in normal cells environment, which was further verified by the observation
that the fluorescence of GFFP-TPPa was significantly quenched compared with
free PPa. Research of cellular morphological variation, uptaking test, and
intracellular single oxygen detection has validated the effectiveness of GFFP-TPPa NPs against tumor cells and potential fluorescence imaging function. Moreover, GFFP-TPPa NPs have excellent superparamagnetic properties and FA-targeted property of NPs, which was verified by the tumor cellular uptake of GFFP-TPPa. Finally, the precise deliver of photosensitizer to mitochondria has been proved by Mito-Tracker Green organelle localization. Our research indicated that the fabricated nanosystem could be applied conveniently for PDT based on multitargetability.