Perpustakaan Digital ITB

Formaldehyde (FA), as a reactive carbonyl species, is extremely hazar dous to human health if its concentration is above normal level. In live cells, lysosome is a main organelle to generate endogenous FA. Thus, the design of facile, stable, and sensitive probes for the detection of FA in lysosome is essential. Herein, a self-assembled fl uorescent nanoprobe based on homoallylamino substituted perylene (P- FA) has been developed for FA detection in lysosome. P-FA can react with FA along with emission color change from blue to green. P-FA exhibited high sensitivity and selectivity to FA in DMSO solution. In aqueous solution, P-FA self-assembled into uniform sphere-like nanoparticle as a fl uorescent nanoprobe. Furthermore, the reaction between the nanoprobe and FA was greatly facilitated at pH 4 ? 5, which led to a lower detection limit (0.96 ? M at pH 5) than that in DMSO. In live cells, P-FA nanoprobe achieved long-term tracking of lysosome (over 12 h). The fl uorescent nanoprobe was then used for both exogenous and endogenous FA detection. Our work provides a facile and e ff ective strategy for the detection of FA in lysosome.