2018_EJRNL_PP_Zhuqing_Li_1.pdf
Terbatas Latifa Noor
» ITB
Terbatas Latifa Noor
» ITB
In tissue engineering scaffolds, macrophages play
a critical role in determining the host response to implanted
biomaterials. Macrophage phenotype is dynamic throughout the
host response, and a balance of phenotypes is essential for timely
progression from injury to proper wound healing. Therefore, it is
important to predict how materials will modulate the response of
macrophages. In this study, we investigated the effect of
methacrylated gellan gum (GG) hydrogels on macrophage
phenotype and proliferation with the ultimate goal of improving
rational design of biomedical implants. Naiv? e, along with
classically and alternatively activated RAW 264.7 macrophages
were seeded on methacrylated gellan gum hydrogels that were
fabricated with different thiol?ene ratios and cross-linking
mechanisms. Live/dead assays showed that all hydrogels supported cell attachment and proliferation. Stiffer substrates
enhanced anti-inflammatory production of nitrites from both naiv? e and classically activated macrophages compared to the softer
substrates. Moreover, arginine and CD206 expression?markers for alternatively activated macrophages?were inhibited by
higher thiol content. Introducing ionic cross-links using calcium did not influence the proliferation or polarization for any of the
three macrophage phenotypes. Our results suggest that the macrophage phenotype shift from M1 to M2 is controlled by the
different cross-linking mechanisms, physical properties, and the chemistry of methacrylated gellan gum hydrogels