Perpustakaan Digital ITB

Virgin coconut oil (VCO) consists highly saturated and medium chain fatty acid, which potentially used for total parenteral nutrition (TPN), by formulating it as microemulsion. Factors affecting globule size and hemolytic activity are important issues in microemulsion for TPN, which were studied in this current research. Factors evaluated include impeller types, preparation size, VCO source variation, soy lecithin preparation, and sterilization method. Hemolytic activity was studied using in vitro static and dynamic method on VCO microemulsion, 2-1 admixture (dextrose 40% addition), and 3-1 admixture (dextrose 40%, and amino acid 10% addition). Globule size measured at day 14 was 57.37 ± 13.22 nm (50 mL) and 70.40 ± 2.41 nm (100 mL) for 2-blade impeller; 403.17 ± 43.73 nm (50 mL), 65.40 ± 9.57 nm (100 mL) for 4-propeller impeller; 70.40 ± 2.41 nm (VCOI); 72.83 ± 2.31 nm (VCOII). Immediate and overnight soaking of soy lecithin did not significantly (p>0.05) affect the globule size. Steam sterilization increased the globule size (79.93 ± 23.77 nm) compared to before sterilization (52.37 ± 4.04 nm), whereas filtration sterilization did not influence the globule size (54.00 ± 5.72 nm). Increase of globule size due to steam sterilization was still in acceptable microemulsion range (10-200 nm). Single VCO microemulsion showed higher hemolytic activity (83.39%) compared to admixture preparations. 2-blade impeller was more suitable for small scale preparation (50-100 mL). Steam sterilization is recommended for VCO microemulsion. VCO microemulsion showed high hemolytic activity.