Perpustakaan Digital ITB

Solid lipid nanoparticles (SLN) are typically colloidal lipid suspensions in which the active compounds entrapped in the lipid core while stabilized by surfactants or polymer. This system can be used for enhancing the oral absorption of low solubility and low permeability of drug (e.g. BCS Class IV) such as Furosemide. This study was aimed to formulate a potential stable oral Furosemide SLN with good physicochemical characteristics. The methods of preparation are high shear homogenization (HSH) combined with ultrasound techniques. Different variables including lipid concentration, surfactant concentration, duration and rotation per minute (RPM) of high shear homogenization (HSH), duration and amplitude of ultra-sonication were optimized to obtain SLN with particle size ranging from 100 to 200nm, low polydispersity index (PDI) and high entrapment efficiency (%EE). Evaluations such as zeta potential, morphology of particles, stability test and in-vitro release profile were carried out on the optimized formulations. The best formula of Furosemide SLN consists of 4%W/V glyceryl monostearate (GMS) and 2%W/V stearic acid as the solid lipid while 3%V/V Tween 80 and 1%V/V Poloxamer 188 as stabilizer. It was stable up to 2 weeks at ???????(under stress condition by temperature) with particle size less than 200nm. Morphology of the SLN under transmission electron microscope (TEM) were observed to be spherical in shape and exhibited less than 10% cumulative drug release at pH1.2 medium for 2 hours, 79.11% in pH7.4 medium in a period of 5 hours. The optimum SLN was generated and has a potential as candidate for oral route dosage from.