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Application of Novel Sialoglyco Particulates Enhances the DetectionSensitivity of the Equine Influenza Virus by Real-Time ReverseTranscriptase Polymerase Chain Reaction

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Penulis : Makoto Ogata, [-]
Kontributor / Dosen Pembimbing :
  • Takashi Yamanaka,Ami Koizumi,Mao Sakamoto,Rena Aita,Hiroyuki Endo,Takehiro Yachi,Noriko Yamauchi,Tadamune Otsubo,Kiyoshi Ikeda,Tatsuya Kato,Enoch Y. Park,Hiroyuki Kono,Manabu Nemoto,Kazuya I. P. J. Hidari
Jenis Koleksi : Jurnal elektronik
Tahun Terbit :
Penerbit : Kimia
Fakultas : Fakultas Matematika dan Ilmu Pengetahuan Alam
Subjek : Chemistry
Kata Kunci : carbohydrate, equine influenza virus, multivalency, particulate, sensitive detection
Sumber : ACS Appl. Bio Mater.XXXX, XXX, XXX?XXX
Staf Input/Edit : Latifa Noor  
File : 1 file
Tanggal Input : 08 Mar 2019

Sialoglyco particulates carrying anN-glycolyl-neuraminyl-?-(2?3)-N-acetyllactosamine (Neu5Gc?2,3-LacNAc) residue that displays a high level of affinity for theequine influenza virus (EIV) were generated using sialogly-copolypeptide and hexyl-containing hybrid silica particulates.The particulates were spherical with a diameter ofapproximately 950 nm and found to have good dispersibilityin aqueous solution. Interaction between the sialoglycoparticulates and the EIV was investigated by real-time reversetranscriptase polymerase chain reaction (rRT-PCR) of theEIV genome captured on the particulates. The number ofEIV-specific genes detected by rRT-PCR on a nasal swab obtained from infected horses clearly increased when the sample wastreated with sialoglyco particulates. Our results show these novel sialoglyco particulates can be used as a highly sensitive tool fordetecting low levels of EIV that were previously undetectable in the early or late stage of infection.

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