Perpustakaan Digital ITB

Diabetes mellitus is a metabolic disorder that characterized by high blood glucose level. Mimosa diplotricha C. Wright ex Sauvalle synonym with Mimosa invisa Martius was traditionally used to treat diabetes mellitus. Therefore, the research was done to investigate the anti-diabetic activity of ethanol extract of Mimosa invisa leaves on alloxan-induced diabetic mice to provide the rationale for the use of Mimosa invisa leaves as traditional anti-diabetic agent. Diabetes was induced on Swiss Webster female mice with 45 mg/kg bw alloxan monohydrate intravenously. Four doses of extract were tested i.e., 10, 20, 50 and 100 mg/kg bw. The parameters that been determined were blood glucose level and the antioxidant activity through DPPH test. After 14 days of orally administration of the extract, the low dose i.e., 10 and 20 mg/kg bw, the extract showed no anti-diabetic effect while at high dose i.e., 50 and 100 mg/kg bw showed anti-diabetic activity by decrease blood glucose level very significantly (p<0.01) started from day of administration. The percentage reduction of blood glucose level for extract at high dose 50 and 100 mg/kg bw were 54.7 and 56.2 % respectively. Based on DPPH test, the extract showed antioxidant activity with IC50 31.3 ppm. The ethanol extract of Mimosa invisa leaves at dose 50 and 100 mg/kg bw were equivalent with dried leaves powder 1.8 and 3.6 g for human calculated based on water content, had anti-diabetic effect on alloxan-induced diabetic mice.