2018_EJRNL_PP_Marta_d_Amora_1.pdf
Terbatas Latifa Noor
» ITB
Terbatas Latifa Noor
» ITB
2018_EJRNL_PP_Marta_d_Amora_2.pdf
Terbatas Latifa Noor
» ITB
Terbatas Latifa Noor
» ITB
This work rationalizes the scalable synthesis of ultrasmall, ligand-free
silicon nanomaterials via liquid-phase pulsed laser ablation process using picosecond
pulses at ultraviolet wavelengths. Results showed that the irradiation time drives
hydrodynamic NP size. Isolated, monodisperse Si-NPs are obtained at high yield (72%)
using post-treatment process. The obtained Si-NPs have an average size of
?
10 nm (not
aggregated) and display photoemission in the green spectral range. We directly
characterized the ligand-free Si-NPs in a vertebrate animal (zebra
fi
sh) and assessed their
toxicity during the development.
In vivo
assay revealed that Si-NPs are found inside in all
the early life stages of embryos and larvae growth, showing that the biosafety of Si-NPs
and malformation types are independent of the Si-NP dose. Si-NPs were directly imaged
inside developing embryos by spinning disk-imaging technique with optical sectioning
capability. We showed that Si-NPs can passively enter inside embryos by the pore canals
of chorion, can di
ff
use in the circulatory system, i.e., blood vessel, and accumulate inside
larvae midgut and yolk sac, and in the eye lens, indicating the crossing of the blood
barrier