Perpustakaan Digital ITB

Rheumatoid arthritis (RA) is an autoimmune disorder that affects primarily the joints and is characterized by chronic inflammation and synovial hyperplasia, pannus tissue which leading to the destruction of cartilage and bone. Sun bear gallbladder (Xiong Dan in Chinese) and cobra snake gallbladder (She Dan in Chinese) contain an appreciable fraction of different types of bile acids have been used widely in Traditional Chinese Medicine (TCM) in treating variety of diseases. The aim of this study was to demonstrate the anti- rheumatoid arthritis activity of sun bear and cobra snake biles on female Wistar rats. Female rats were previously induced to have RA by subcutaneously injected with bovine type II collagen emulsified in Complete Freund’s Adjuvant (CFA), followed by a booster dose of bovine collagen type II emulsified in Incomplete Freund’s Adjuvant (IFA) injected through tail. Sun bear and cobra snake biles were prepared by dissolving biles in water. The CIA rats were divided into 6 groups which are control group (water only), test groups: sun bear bile at dose 0.225 and 0.1125 g/kg; cobra snake bile at dose 0.27 and 0.135 g/kg and methylprednisolone 45mg/kg as reference group. All the rats in each group were given orally for 21 days. The parameters determined were macroscopic scoring, TNF-?and histopathological study of rat hindlimb tissues. Based on the data of arthritis score, sun bear bile at dose 0.225 and 0.1125 g/kg and snake bile at dose 0.27 and 0.135 g/kg can decreased arthritis score significantly (p<0.05). There is no significant difference of arthritis score after treated with standard (methylprednisolone) with all the treatment groups. In determination of TNF-?level, sun bear bile at dose 0.225 and 0.1125 g/kg and snake bile at dose 0.27 g/kg showed a significant reduction of TNF-?(p<0.05) as compared to control group. Histological analysis showed sun bear bile at dose 0.225 and 0.1125 g/kg and cobra snake bile at dose 0.27 g/kg have ability to protect cartilage from erosion. Sun bear bile at dose 0.225 and 0.1125 g/kg and cobra snake bile at dose 0.27 g/kg have anti-rheumatoid arthritis effect in CIA rat model.