Perpustakaan Digital ITB

Skin microbiome has become an interesting topic because of the importance of it towards our skins. The distruption of skin microbiome could lead to various skin’s problems such as exim because of the domination of Staphyloacoccus aureus and acnes because of Propionibacterium acnes. Probiotics could be the new solution to maintain our skin microbiome while also benefits our skin health and beauty because of it’s abilites to produce metabolites which are beneficial for us. Lactobacillus plantarum could produce organic acids which could rehydrate and exfoliate our skin cell, bacteriocin that could inhibit growth of pathogenic bacteria and has antioxidant activites that could protect our skin from radical matters. In this research, we would like to understand the potential of L. plantarum who cultured in milk to produce metabolites that could inhibit the growth of pathogenic bacteria thus it could be used for cosmetic’s active compounds. The evaluation of the inhibition activites in the preliminary studies was conducted from extracellular metabolites, intracellular metabolites, and the cell of L. plantarum by Kirby-bauer methods using S. aureus and E. coli as the test subject. After that, optimation was conducted with pasteurized milk pH 6.5 for 48 hours, 37 °C. The variation of inocula that was used were 5%(I5), 10%(I10), 15%(I15). The total organic acids were observed every 4 hours and the inhibition activites was observed every 8 hours using s. aureus and P. aeruginosa as test subject. The result of preliminary test shows the inhibition activities of extracellular metabolite presence at 12 hours and reach it peak at 48 hours with diameter of the zone of inhibition for S. aureus is 8.43±0, 24mm and E. coli is 9.89±0.55mm. The intracellular metabolite has the inhibition activites at S aureus at 48 hours with diameter of the zone of inhibition of 2.78±3.93mm and for the cell of the L. plantarum did not show any sign of inhibition activites. Optimation of cell concentration in pasteurized milk with variation of I5, I10, and I15 produce growth rate of cell at 0, 188/hr, 0, 202/hr, and 0, 24/hr, Total organic acids at 16.041ppm/hr, 20.883ppm/hr, 24, 167ppm/hr, and the decrease of pH at 0.051/hr, 0.057/hr, 0.055/hr respectively. The result of inhibition activites of S. aureus presence at 24hr for the I5 and I10 but for I15 variation it also presence at 16hr with the zone of inhibition of 7±0.0mm, 7.83±0, 25mm, and 6.04±0.6mm respectively. For the inhibition activites of P. aeruginosa, it presence at 48hr for I5 variation, 40hr for I10, and 16 hr for I15 with the zone of inhibition of 6.94±0.76mm, 5.3±0.31mm, and 6.99±0.014mm respectively. The conclusion of this research shows that L. plantarum which cultured with pasteurized milk has the potential to be used as a cometic active compounds with the optimum inocula of 15% because it shows the inhibition effect of S.aureus at 16hr (6.99±0.014mm) and P,aeruginosa at 16hr (6.04±0.6mm)