2020 EJRNL PP Swati Kaushik - 1.pdf
Terbatas Irwan Sofiyan
» ITB
Terbatas Irwan Sofiyan
» ITB
Despite the promising role of magnetic hyperthermia in
cancer therapy, its use in patients has been restricted by hurdles that
include inefficient targeting of magnetic particles to the tumor site,
limited bioavailability, and high toxicity, etc. Taking advantage of the
unique metabolic property of cancer cells, we explored the potential of
these cells to biosynthesize magnetic nanoparticles for potential
hyperthermia applications. Treatment of cancer cells with a mixture of
FeCl2 and zinc gluconate resulted in a significant increase in intracellular
Fe and Zn content in these cells. Exposure of these cells to an alternating
magnetic field (AMF) for 30 min resulted in a substantial temperature
rise of 5?6 °C. The in situ formed particles were identified as iron oxide
and ZnO nanoparticles. Based on the magnetic property and size, the
iron oxide nanoparticles were classified as superparamagnetic iron oxide
nanoparticles (SPIONS) comprising a mixture of magnetite (Fe3-?O4) and maghemite (?-Fe2O3). The role of reactive oxygen species (H2O2) and the involvement of the glycolytic pathway in the biosynthesis of the nanoparticles were confirmed using appropriate in vitro studies. The simplicity of treatment, the specificity of cells capable of synthesis of SPIONS, and the hyperthermia response observed in cancer cells indicate a promising strategy to achieve effective magnetic hyperthermia for cancer therapy.