2020 EJRNL PP Jun Ohata -1.pdf
Terbatas Irwan Sofiyan
» ITB
Terbatas Irwan Sofiyan
» ITB
Carbon is central to the chemistry of life, and in addition to
its fundamental roles as a static component of all major biomolecules spanning
proteins, nucleic acids, sugars, and lipids, emerging evidence shows that small
and transient carbon-based metabolites, termed reactive carbon species
(RCS), are dynamic signaling/stress agents that can influence a variety of
biological pathways. Recent examples include the identification of carbon
monoxide (CO) as an ion channel blocker and endogenous formaldehyde
(FA) as a one-carbon metabolic unit formed from the spontaneous
degradation of dietary folate metabolites. These findings motivate the
development of analytical tools for transient carbon species that can achieve
high specificity and sensitivity to further investigate RCS signaling and stress
pathways at the cell, tissue, and whole-organism levels. This Account
summarizes work from our laboratory on the development of new chemical
tools to monitor two important one-carbon RCS, CO and FA, through
activity-based sensing (ABS), where we leverage the unique chemical
reactivities of these small and transient analytes, rather than lock-and-key
binding considerations, for selective detection. Classic inorganic/organometallic
and organic transformations form the basis for this approach....