2011 EJRNL PP Chen Xi 1.pdf?
Terbatas Irwan Sofiyan
» ITB
Terbatas Irwan Sofiyan
» ITB
Spatial isolation is currently thought to represent one of the major factors resulting in bacteria genetic
variation and population abundance. The bacterial diversity in a distinct environment Zoige Alpine Wetland
located in the northeast of the Qinghai-Tibetan Plateau with the altitude 3400 m on average aroused
our great attention. This area belongs to Qinghai-Tibetan cold climate zone with the mean annual temperature
about 1 C. Although several studies on bacterial diversity in Qinghai-Tibetan Plateau had been
reported, there is no report on wetland water in this area. In this work, six water samples were collected
and the water qualities including CODCr, NHþ4
-N; NO
3 -N; NO
2 -N, TN, TP, TOC were investigated, of which
results indicated that more than 80% samples sorted as II–V class of surface water sources according to
the National Water Quality Standard of China (GB3838-2002). Comparison of bacterial communities
among the six samples was analyzed by DGGE of PCR-amplified 16S rDNA with universal bacterial primer
sets. The profiles demonstrated that samples from the Flower Lake had more DNA bands than the Conservatory Station inferring higher diversity. In addition, the samples from the same environment shared
similar compositions of bacterial communities. Bacterial community composition and predominant bacteria
were analyzed by 16S rDNA clone library. The dominant group was Proteobacteria (51.6% of the total
clones, which contained 24.2% alpha proteobacteria, 14.5% beta proteobacteria and 12.9% gamma proteobacteria). And the Bacteroidetes added to 17.7%, Verrucomicrobia to 4.8%. More than 24.2% of the total clones showed high similarity to uncultured bacteria. The above work provides some information on bacterial diversity for special site of spatial isolation.