2019_EJRNL_PP_FUZHENG_ZHAO_1.pdf
Terbatas Lili Sawaludin Mulyadi
» ITB
Terbatas Lili Sawaludin Mulyadi
» ITB
Due to complexity and variety of pharmaceutical wastewater composition, little is known as for functionally important
microflora of pharmaceutical wastewater treatment plants (pWWTPs).We compared bacterial composition and diversity of pWWTPs (27 sludge samples collected from 12 full-scale pWWTPs) with those of other industrial (iWWTPs) (27
samples) and municipal wastewater treatment plants (mWWTPs) (27 samples) through meta-analysis based on 16S
rRNA gene amplicon sequencing, and identified putatively important organisms and their ecological correlations. Nonmetric multidimensional scaling indicated that the pWWTPs, iWWTPs and mWWTPs showed distinctive differences in
bacterial community composition (P b 1e?04), and the pWWTPs had significantly lower bacterial diversity than the
mWWTPs (P b 1e?06). Thermotogae and Synergistetes phyla only strictly dominated in the pWWTPs, and 26, 30
and 6 specific genera were identified in the pWWTPs, mWWTPs and iWWTPs, respectively. Totally, 15 and 1300
OTUs were identified as core and occasional groups, representing 23.2% and 66.2% of the total read abundance of the
pWWTPs, respectively. Permutational multivariate analysis of variance revealed that the bacterial components were
clearly clustered corresponding to the types of pharmaceutical wastewater, and a total of 129 local specific OTUs
were identified in the pWWTPs, among which anticancer antibiotics pWWTPs had the highest number of specific
OTUs (40 ones). Co-occurrence network revealed that the species dominating in the same type of pWWTPs tended
to co-occur much more frequently than theoretical random expectation. The results may extend our knowledge regarding the ecological status and correlation of the key microflora in pWWTPs.