Perpustakaan Digital ITB

Heparin is a naturally occurring sulfated glycosaminoglycan polysaccharide often used as an anticoagulant agent. In this work, a series of water swellable cross-linked polymer networks consisting of acrylamide (AAm) as a support, N,N?- methylene bis(acrylamide) (BisAAm) as a cross-linker, and (3-acrylamidopropyl)-trimethylammonium chloride) (APTMAC) as a heparin adsorbent was synthesized in water at different ratios of AAm to APTMAC in one-pot. A two-step polymerization process was used to incorporate APTMAC as an adsorbent primarily on the surface of the preformed but still growing crosslinked AAm support (X-AAm). Heparin adsorption results showed that the sample with 15 mol % APTMAC incorporated into the X-AAm core demonstrated efficiency as high as the 100% APTMAC resin, with a high adsorption capacity of 223.36 mg g?1 , which makes it the sample of choice for industrial application and cost-effectiveness. The adsorption efficiency of the optimized sample, X-AAm-APTMAC-(85?15) (that contains 15 mol % APTMAC), was evaluated versus some of the operational variables, including adsorbent dosage, initial heparin concentration, temperature, pH, and time as well as the stability and reusability of the resin, which showed satisfying performance in a wide range of operational parameters with suitable stability and reusability. Heparin was efficiently adsorbed by the X-AAm-APTMAC-(85?15) from a biological sample containing enzymatically digested heparin. The calculated bioactivity of heparin eluted from X-AAm-APTMAC-(85?15) beads and Amberlite FPA98 Cl, an industrially available adsorbent for heparin recovery, was 3430 and 2623 units, respectively. Therefore, the eluted heparin that adsorbed from the biological mixture using synthesized resin complies with the requirement of the United States Pharmacopeia.