2016_EJRNL_PP_S__DUTTA_1.pdf
Terbatas Suharsiyah
» ITB
Terbatas Suharsiyah
» ITB
The bioavailability of organic contaminants to the degrading bacteria is a major
limitation to efficient bioremediation of sites contaminated with hydrophobic pollutants.
Studies were conducted to analyze the presence of biodegrading bacteria on the sites of
refinery and garage oil spills with abundance of hydrocarbons. Contaminated soils were
collected from these rich hydrocarbon sites and bacteria were isolated from samples
using regular bacterial media enriched with petroleum oil. These isolates were
characterized for their efficiency to utilize refining petroleum oil as their energy source
and then the most common isolate was characterized. Further investigations examine the
formation of biofilm by naturally existing oil-degrading bacteria on refine petroleum oil
degradation. Here also studied the abilities of these Pseudomonas strains to respond
chemo tactically to refine petroleum oil. Microbial chemotaxis plays important role for
formation of biofilm. 16S rDNA analysis of the best degrader was found to belong to
the Pseudomonas species. Interestingly, one of the best isolates was found to be close to
Pseudomonas aeruginosa family