Perpustakaan Digital ITB

Plant-derived exosome-like nanoparticles (PDENs) are nano-sized vesicles containing biomolecules that are released by multivesicular bodies. Recently, studies have shown the efficacy of exosomes in treating photoaging, through increasing collagen synthesis and decreasing collagen degradation. In addition to that, some PDENs were also proven to contain bioactive metabolites, which also have potential antioxidant activity to mitigate the risk of photoaging. Thus, this present study aims to produce a novel therapeutic approach for the treatment of photoaging by formulating and developing a gel and incorporating it with exosomes derived from golden cherry (Physalis minima). The formulation was developed by first preparing various base formulations with different compositions, and selecting the best through evaluation test. The results obtained showed only polymer base natrosol with concentration of 0.25% were suitable for the incorporation of exosomes. The selected base was then incorporated with various concentrations of golden cherry exosomes, and was evaluated regarding its physical and stability profile. The result demonstrates that the incorporated gel not only displayed pleasant organoleptic properties, but also shows a pH compatible with skin, with pseudoplastic flow, and a suitable viscosity for topical application. The stability study also only revealed minor changes in viscosity and pH, without affecting the general stability of the formulation. Formulation incorporating 0.25% golden cherry exosomes, had shown the best stability profile compare to other concentrations. On characterization, although the incorporated exosomes showed heterogeneous particle size distribution (PI > 0.3), they still maintained their structural integrity. In addition to that, immunological assay on incorporated exosomes incubated with human dermal fibroblast had showed an increase in type 1 collagen expression. In conclusion, golden cherry exosomes had been successfully incorporated into gel and thus, can be potentially utilized as a novel therapeutic approach for the treatment of photoaging.