2020 EJRNL PP Kenneth Kam -1.pdf
Terbatas Irwan Sofiyan
» ITB
Terbatas Irwan Sofiyan
» ITB
For more than 15 years, bioorthogonal chemistry
has received increasing attention due to its successful applications in
the detection and imaging of biomolecules in their native biological
environments. The method typically proceeds with the incorporation
of a biological substrate appended with a bioorthogonal
functional group (chemical reporter), followed by the introduction
of the substrate to biological systems. Biomolecules containing the
substrate are then recognized by an exogenously delivered
bioorthogonal probe. Despite the fact that many useful chemical
reporters and bioorthogonal reactions have been developed, most of
the bioorthogonal probes reported thus far are fluorescent dyes. A
limitation is that stringent washing is required due to the interference caused by the background fluorescence of unreacted probes. Thus, fluorogenic probes with turn-on emission properties upon bioorthogonal labeling have been designed as an alternative strategy. These probes are highly appealing because excellent images can be obtained without the need for washing steps.