2011 EJRNL PP Zou Yukun 1.pdf?
Terbatas Irwan Sofiyan
» ITB
Terbatas Irwan Sofiyan
» ITB
Biological nitrogen fixation through prokaryotic microbe is an important source of nitrogen been input
into many natural ecosystems. In this study the active diazotrophic community was investigated in
the three treatments of mowed, grazed and enclosed Leymus chinensis steppes in Hulunbeier grassland
of Inner Mongolia by using approaches of polymerase chain reaction-denaturing gradient gel electrophoresis
(PCR–DGGE) and sequence analysis. The community structure and diversity of the bacterial groups
from the different samples was further analyzed by using different techniques, such as statistical analysis
and diversity index evaluation of the band patterns etc. The results showed that grazing activity significantly
reduced the number of species and quantities of nitrogen-fixing microorganisms, as well as the
nifH gene diversity.