2019_TS_PP_MOHAMED_MAHMUD_E__ALDEEB_1-ABSTRAK.pdf
PUBLIC yana mulyana COVER Mohamed Aldeeb
PUBLIC yana mulyana BAB 1 Mohamed Aldeeb
PUBLIC yana mulyana BAB 2 Mohamed Aldeeb
PUBLIC yana mulyana BAB 3 Mohamed Aldeeb
PUBLIC yana mulyana BAB 4 Mohamed Aldeeb
PUBLIC yana mulyana BAB 5 Mohamed Aldeeb
PUBLIC yana mulyana BAB 6 Mohamed Aldeeb
PUBLIC yana mulyana PUSTAKA Mohamed Aldeeb
PUBLIC yana mulyana
The aim of this study was to improve the aqueous solubility of the oral
hypoglycemic agent, glibenclamide, in order to improve its absorption, and
hence its bioavailability after oral administration. Several approaches have been
done to improve the solubility of glibenclamide. One of them is by the
formation of an inclusion complex. In this study, we did the complexation of
glibenclamide with cyclodextrin by comparing two different types of
cyclodextrin, i.e. ?-cyclodextrin (?CD) and 2-hydroxyropyl-?- cyclodextrin
(HP?CD). The complex was prepared by two different methods, i.e. freeze
drying (FD) and coevaporation (CO). The phase solubility studies were
performed for 2 days in phosphate buffer pH 7.4. The obtained solution was
filtered and the glibenclamide concentration was measured by using High-
Performance Liquid Chromatography (HPLC) with UV-Vis detector at the
wavelength of 300 nm. Furthermore, the FD and CO products were prepared by
dissolving ?CD and HP?CD in ammonia-water solution. The reaction was
carried out for 1 day, and the solvents were eliminated by either freeze drying
for FD product or using rotary evaporator for CO product. The dissolution
study was conducted to evaluate the influence of complexation on the
dissolution rate of glibenclamide. Furthermore, Differential Scanning
Calorimetry (DSC), X-ray Powder Diffraction (X-RD), and Fourier-Transform
Infrared Spectroscopy (FTIR) studies were performed to characterize the
powder properties. The highest stability constant was obtained from the
complex prepared using HP?CD, and the highest percentage of dissolved
glibenclamide was obtained from FD product of complexation with HP?CD. In
addition, the DSC, XRD, and FTIR results confirmed the chemical interaction
between glibenclamide and cyclodextrin.