Perpustakaan Digital ITB

In this work, we fabricated a thermosensitive hydrogel based on NIPAM that incorporated an oxygen-permeable hyaluronic acid (HA) polysaccharide unit. The interpenetrating network (IPN) of NIPAM-HA-MA hydrogel was fabricated by using an ultraviolet (UV) cross-linking process. Importantly, we explored the real behavior kinetics of the hydrogel via the mechanical properties of Young ’ s modulus and the shear strain. The mechanical properties were reduced when the temperature was raised from 25 to 35 ° C, and lower temperatures increased the rigidity of the IPN gel. This dynamic change occurred because of NIPAM ’ s thermosensitive character. Further, IPN hydrogel exhibits good transparency with the incorporation of MA-HA and this stimuli-responsive hydrogel was biocompatible with a human dermal fi broblasts cell line (HDF). The cells adherence was densest when the temperature reached 37 ° C and was reduced when the temperature was lowered to 25 ° C. We envision using such hydrogels as mimics of cell-instructive biomaterials in tissue engineering applications.