Path: TopS1-Final ProjectChemistry-FMIPA2015

OPTIMASI MEDIUM PERTUMBUHAN THALASSIOSIRA SP. DAN KONSENTRASI SEL INOKULUM AWAL UNTUK MENINGKATKAN PRODUKTIVITAS LIPID DI LUAR RUANGAN

Undergraduate Theses from JBPTITBPP / 2017-09-27 11:42:38
Oleh : YESTRIA YASWARI (NIM : 10510022), S1 - Department of Chemistry
Dibuat : 2015, dengan 7 file

Keyword : medium, biomass, extraction, productivity.

In order to improve the productivity of microalgal lipid Thalassiosira sp. that was grown in the outdoor photobioreactor, in the present study the optimization of N:P:Si content obtained from various sources for growth medium and the optimization of initial inoculum cell concentration were carried out. In a previous study was reported that Thalassiosira sp. produced lipids that could be used as an alternative of biofuel source. However, the effect of cell concentration on the productivity of biomass and lipids remained unknown. In the present study, the N:P:Si ratio derived from a various fertilizer: Medium 1 (mixture of urea-NaH2PO4-metasilicate), Medium 2 (mixture of Hyponex®-metasilicate), Medium 3 (mixture of Hyponex®-SiPPG fertilizer), Medium 4 (mixture of urea-TSP fertilizer-metasilicate), and Medium 5 (mixtute of urea-TSP fertilizer-SiPPG fertilizer) was optimized for the Thalassiosira sp. growth medium. In order to determine the biomass and oil productivity, the optimization of initial inoculum concentration was performed for act of the planting of the outdoor photobioreactor. In addition, iron content in the growth medium was also further optimized. The results showed that the Thalassiosira sp. cells growth in Medium 1 with a salinity of 25 ppt ( ̅ =the average of specific growth rate=0.592±0.107 per day) was the best condition. The following research showed that Medium 2 was the optimum growth medium which produced the average of specific growth rate of 0.703 per day, respectively. The obtained Thalassiosira sp. lipids from the outdoor culture by using Medium 2 with an initial inoculum cell concentration of 0.1, 0.5, and 1.0 ×106 cells/mL was 2.90, 3.04 and 2.86 × 10-4 g/L culture/day, respectively. Optimization result of iron mineral content in the growth medium showed that Medium 2 without additional Fe (0 mg/L FeCl3) was compatible as the medium growth of Thalassiosira sp. with the specific growth rate of 0.569 per day, respectively. Thus, Medium 2 containing 115 mg/L Hyponex® as a source of 11.8 mg/L nitrogen and 2.5 mg/L phosphorous as well as 10 mg/L metasilicate as a source of 1.3 mg/L silicon, with salinity 25 ppt without additional Fe and an initial inoculum cell concentration of 0.5 × 106 cells/mL was the best condition to produce Thalassiosira sp. biomass.

Deskripsi Alternatif :

Untuk meningkatkan produktivitas lipid mikroalga Thalassiosira sp. yang ditumbuhkan dalam fotobioreaktor di luar ruang, pada penelitian ini optimasi N:P:Si berbagai sumber untuk medium pertumbuhan dan optimasi konsentrasi sel inokulum awal dilakukan. Pada penelitian terdahulu dilaporkan bahwa Thalassiosira sp. menghasilkan lipid yang dapat digunakan sebagai alternatif sumber bahan bakar. Namun, pengaruh konsentrasi sel terhadap produktivitas biomassa dan lipid belum diketahui. Pada penelitian ini, perbandingan N:P:Si yang berasal dari sumber pupuk urea-NaH2PO4-metasilikat (Medium 1), pupuk Hyponex®-metasilikat (Medium 2), pupuk Hyponex®-pupuk SiPPG (Medium 3), pupuk urea-pupuk TSP-metasilikat (Medium 4), dan pupuk urea-pupuk TSP-pupuk SiPPG (Medium 5) dioptimasi untuk medium pertumbuhan Thalassiosira sp. Untuk mengetahui produktivitas biomassa dan minyak, variasi konsentrasi inokulum awal dilakukan untuk penanaman dalam fotobioreaktor di luar ruangan. Selain itu, kandungan mineral besi dalam medium pertumbuhan juga dioptimasi lebih lanjut. Hasil penelitian menunjukkan bahwa pertumbuhan sel Thalassiosira sp. yang ditanam dalam Medium 1 dengan salinitas 25 ppt adalah paling baik dengan laju pertumbuhan sel spesifik rata-rata sebesar 0,592±0,107 per hari. Hasil penelitian berikutnya menunjukkan bahwa Medium 2 adalah medium optimum yang menghasilkan laju pertumbuhan spesifik rata-rata 0,703 per hari. Lipid Thalassiosira sp. yang dihasilkan dari kultur luar ruangan menggunakan Medium 2 dengan konsentrasi sel inokulum awal 0,1, 0,5, dan 1,0 ×106 sel/mL berturut-turut sebesar 2,90, 3,04 dan 2,86 ×10-4 g/L kultur/hari. Hasil optimasi kandungan mineral besi dalam medium pertumbuhan menunjukkan bahwa Medium 2 tanpa Fe tambahan (0 mg/L FeCl3) cocok sebagai medium pertumbuhan Thalassiosira sp. dengan laju pertumbuhan spesifik rata-rata sebesar 0,569 per hari. Jadi, Medium 2 yang terdiri dari pupuk Hyponex® (115 mg/L) sebagai sumber N (11,8 mg/L) dan P (2,5 mg/L), metasilikat (10 mg/L) sebagai sumber Si (1,3 mg/L), serta Na2EDTA 70 mg/L, dengan salinitas 25 ppt tanpa tambahan sumber Fe dan konsentrasi sel inokulum awal 0,5 ×106 sel/mL merupakan kondisi terbaik untuk memroduksi biomassa Thalassiosira sp.

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OrganisasiS1 - Department of Chemistry
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NegaraIndonesia
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  • Pembimbing : Prof. Zeily Nurachman, D.Sc, Editor: Alice Diniarti

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