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ANALISIS DNA TURUNAN GENERATIF JATI
(Tectona grandis U) DENGAN METODA RANDOM AMPLIFIED POLYMORPHIC DNA

THE DNA ANALYSIS OF TEAK PROGENIES (Tectona grandis L.f) USING THE RAPD METHOD

Master Theses from #PUBLISHER# / 2006-03-07 10:52:03
Oleh : Imas Cintamulya, S2 - Biology
Dibuat : 1998-03-00, dengan 1 file

Keyword :
DNA Analysis-Random Amplified polynorphic DNA, Cluster analysis

Subjek :
Biochemical genetics-DNA-Deoxiribonucleid acid

Kepala Subjek :
Biochemistry

Nomor Panggil (DDC) : T 572.86 CIN
Sumber pengambilan dokumen : 20060486

Abstrak

align="justify">Telah dilakukan analisis DNA dari turunan generatif pohon jati dengan menggunakan metoda Random Amplified Polymorphic DNA (RAPD). Analisis dilakukan untuk mengetahui persamaan dan perbedaan antara turunan generatif dengan pohon jati induk pada tingkat DNA. Sampel DNA berasal dari pohon jati "plus" No. 24 dan 30 serta turunan generatifnya. Sampel DNA diamplifikasi dengan menggunakan 7 macam primer berukuran 10 nukleotida yang selanjutnya dielektroforesis path gel agarosa 1,4 %. Persamaan "Simple Maching" digunakan untuk menghitung koefisien kesamaan antara turunan generatif dan pohon jati induk. Data matrik kesamaan dipakai untuk membentuk dendogram dengan menggunakan UPGMA (Unweighted Pair-Group Method with Arithmetic Averages). Hasil amplifikasi ketujuh primer memperlihatkan lank DNA yang berjumlah 114 dengan ukuran berkisar antara 200-3610 pb. Dari sampel DNA pohon jati "plus" No. 24 dan turunan generatifnya ditemukan 3 lank DNA spesifik dengan panjang masingmasing 460, 640 dan 1400 pb. Sementara itu dari sampel DNA pohon jati "plus" No. 30 dan turunan generatifnya tidak ditemukan lank DNA spesifik. Koefisien kesamaan antara pohon jati "plus" No.24 dan turunan generatifnya berkisar antara 0,58-0,84 dan pada pohon jati "plus" No.30 dan turunan generatifnya berkisar antara 0,62-0,86. Kemudian dari analisis klaster diperoleh dua kelompok yang memisahkan turunan generatif pohon jati "plus" No.24 dan induknya demikian juga dengan turunan generatif pohon jati "plus" No. 30 dan induknya. Dari hasil analisis pada tingkat DNA dapat dilihat, bahwa turunan generatif pohon jati "plus" belum tentu sama secara genetik dengan induknya. Demikian juga diantara sesama human generatif pohon jati "plus" menunjukkan adanya keragaman genetik.

Deskripsi Alternatif :

Abstrack

align="justify">
The DNA analysis was carried out to evaluate progenies of teak trees by using the RAPD (Random Amplified Polymorphic DNA) method. The analysis was done to compare progenies and their parentals of teak at the DNA level. DNA samples were isolated from the "plus" teak trees (No.24 and 30) and their progenies. DNA samples were amplified by using 7 different nucleotide decamer primers and separated by electrophoresis on 1.4% agarose gels. "Simple Matching" equation was used to determine similarity coefficient between progenies and their parentals. A dendogram was constructed based on the matrix data by applying unweighted pair-group method with arithmetic averages (UPGMA). The result showed that there were 114 DNA bands ranged between 200-3610 bp. There were three specific bands of 460, 640 and 1400 bp in size from DNA samples of "plus" teak trees No. 24 and their progenies. There was no specific band from DNA samples of "plus" teak trees No. 30 and their progenies. Similarity coefficient of "plus" teak trees No. 24 and their progenies ranged between 0,58-0,84 and "plus" teak trees No. 30 and their progenies ranged between 0,62-0,86. The result of cluster analysis showed that there were two main groups of the family of each "plus" teak tree and its progenies. One group is the group of progenies and the other group is the parental. The experimental results indicated that, at the DNA level, progenies of "plus" teak trees were not always the same, genetically, compared to their parentals. And so, among progenies from the same "plus" teak trees showed a genetic diversity.

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  • Pembimbing:
    Sri Nanan B. Widiyanti, Dr.

    Scan:
    Hidayat (2006-03-07)

    , Editor: hidayat@#publisher#

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