Path: TopS1-Final ProjectChemistry-FMIPA2017

Penapisan Koleksi Bakteri Pendegradasi Asam Monokloroasetat dan Identifikasi Bakteri Menggunakan Ribotyping

Undergraduate Theses from JBPTITBPP / 2017-09-27 11:42:40
Oleh : Fonna Dista Maisa Pusporini , S1 - Department of Chemistry
Dibuat : 2017-09-20, dengan 1 file

Keyword : Degradasi, organohalogen, Bergmann dan Sanik, MCA, sumber karbon, glukosa

Organohalogen merupakan golongan senyawa yang banyak digunakan sebagai bahan plastik, PVC, obat, pestisida dan herbisida. Namun senyawa ini bersifat xenobiotik, persisten di lingkungan dalam waktu yang cukup lama. Akumulasi organohalogen secara berlebihan dalam tubuh manusia dapat menyebabkan antara lain kerusakan fungsi hati, ginjal, sistem syaraf, serta menimbulkan cacat bawaan. Salah satu senyawa organohalogen yang banyak digunakan adalah asam monokloroasetat. Penelitian terhadap bakteri pendegradasi asam monokloroasetat dilakukan untuk mendukung bioremediasi polutan asam monokloroasetat di lingkungan. Penelitian ini menguji kemampuan beberapa bakteri strain lokal, yakni, Pseudomonas aeruginosa 1, Pseudomonas aeruginosa 2, Pseudomonas fluorescens, Azotobacter chroococcum, Bacillus cereus, dan Staphylococcus aureus untuk mendegradasi asam monokloroasetat (MCA) di dalam Minimal Medium (MM). Keenam bakteri tersebut mampu tumbuh dalam medium LB yang mengandung 0,5 mM MCA. Uji degradasi MCA dilakukan menggunakan metode Bergmann dan Sanik dengan mengukur kadar Cl⁻ yang dilepaskan ke medium. Dalam MM tanpa glukosa, Pseudomonas aeruginosa 1 mampu mendegradasi 18 mM MCA sebanyak 2,04%, Pseudomonas aeruginosa 2 mampu mendegradasi 12 mM MCA sebanyak 3,59%, dan Pseudomonas fluorescens mampu mendegradasi 15 mM MCA sebanyak 0,48%, sedangkan Azotobacter chroococcum, Bacillus cereus, dan Staphylococcus aureus tidak dapat mendegradasi MCA. Dalam MM yang mengandung 0,005% (w/v) glukosa, Azotobacter chroococcum mampu mendegradasi 8 mM MCA sebanyak 2,86%, dan Bacillus cereus mampu mendegradasi 5 mM MCA sebanyak 9,25%. Dalam MM yang sama, Staphylococcus aureus membutuhkan 0,01% (w/v) glukosa untuk mendegradasi 5 mM MCA sebanyak 4,43%. Identifikasi ribotyping dilakukan untuk mengkonfirmasi spesies bakteri yang mendegradasi MCA paling tinggi, dengan hasil bahwa Pseudomonas aeruginosa 1 dan Pseudomonas aeruginosa 2 adalah benar Pseudomonas aeruginosa, sedangkan Pseudomonas fluorescens adalah Limnobacter sp.

Deskripsi Alternatif :

Organohalogen is a compound that is commonly used to produce plastics, PVC, medicines, pestisides, and herbicides. Organohalogen is classified as xenobiotic, and persistent in the environment for a long time. Excessive accumulation of organohalogen in human body can cause liver dysfunction, kidneys dysfunction, nerve system malfunction, and even congenital defects. One of most used organohalogen is monochloroacetic acid (MCA). Researchs of monochloroacetic acid degrading bacteria was done to support MCA bioremediation. Some local strain bacteria was screened for its activity to degrade MCA, that is Pseudomonas aeruginosa 1, Pseudomonas aeruginosa 2, Pseudomonas fluorescens, Azotobacter chroococcum, Bacillus cereus, dan Staphylococcus aureus using MCA as the substrate in Minimal Medium (MM). These bacteria were able to grow in LB medium that contains 0,5 mM MCA, which indicates that these bacteria can tolerate the presence of MCA. MCA degradation test was conducted with Bergmann and Sanik method to quantify the concentration of Cl⁻ released to the medium. In MM that does not contain glucose, Pseudomonas aeruginosa 1 was able to degrade 2,04% of 18 mM MCA, Pseudomonas aeruginosa 2 was able to degrade 3,59% of 12 mM MCA, and Pseudomonas fluorescens was able to degrade 0,48% of 15 mM MCA, while Azotobacter chroococcum, Bacillus cereus, and Staphylococcus aureus was not able to degrade MCA. In MM that contains 0,005% (w/v) glucose, Azotobacter chroococcum was able to degrade 2,86 % of 8 mM MCA and Bacillus cereus was able to degrade 9,25% of 5 mM MCA. In the similar MM, Staphylococcus aureus needed 0,01% (w/v) glucose to degrade 4,43% of 5 mM MCA. Ribotyping identification was conducted to confirm the species of the highest MCA degrading bacteria, Pseudomonas aeruginosa 1 and Pseudomonas aeruginosa 2 is Pseudomonas aeruginosa, while Pseudomonas fluorescens is Limnobacter sp.

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