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PENGEMBANGAN FORMULA ASAM FOLAT DALAM SOLID LIPID NANOPARTICLE
By : DEVI FAJAR SUHENDAR (NIM 10704109), S1 - Department of Pharmacy
Created : 2008, with 8 files
Keyword : asam folat, solid lipid nanoparticle, neonatal infant
Asam folat merupakan salah satu zat yang penting untuk pertumbuhan bayi, yaitu untuk mencegah terjadinya neural tube defect (NTD), yang dapat menyebabkan cacat otak dan sum-sum tulang pada bayi yang baru lahir. Asam folat dalam sediaan memiliki tingkat kestabilan yang rendah bahkan dalam suhu kamar, sehingga perlu peningkatan kestabilan dengan cara pembentukan solid lipid nanoparticle (SLN) atau nanopartikel lipid padat. Tujuan dari penelitian ini adalah mengembangkan formula SLN untuk mengenkapsulasi asam folat. SLN asam folat dibuat dengan metode mikroemulsi. Asam lemak padat yang digunakan adalah asam palmitat, surfaktan digunakan Cremophor RH-40 dan ko-surfaktan etanol. Optimasi formula dilakukan terhadap perbandingan asam folat dan asam palmitat serta jumlah surfaktan (cremophor RH-40) dan ko-surfaktan (etanol), yang kemudian disolidifikasi dalam aquadest dingin untuk membentuk partikel yang lebih halus. Karakterisasi SLN meliputi morfologi, ukuran dan distribusi ukuran partikel. Morfologi SLN ditentukan dengan alat Scanning Electron Microscope (SEM). Distribusi ukuran partikel ditentukan menggunakan alat Particle Size Distribution Analyzer. Asam folat yang terjerat dalam SLN ditentukan dengan Kromatografi Cair Kinerja Tinggi (KCKT). Formula mikroemulsi yang paling baik menghasilkan mikroemulsi transparan dengan ukuran partikel yang sferis dan sesuai dengan ukuran SLN (50-1000 nm) adalah mengandung 0.015% asam folat, 20% Cremophor RH-40, 10% etanol dan 2% asam palmitat. Distribusi ukuran partikel SLN adalah 259-877 nm dengan efisiensi penjeratan asam folat dalam SLN adalah 17.28%. Pembentukan SLN dipengaruhi oleh beberapa faktor yaitu jenis lemak padat dan surfaktan, perbandingan jumlah lemak padat, surfaktan dan obat dalam mikroemulsi, serta proses solidifikasi.
Description Alternative :Folic acid is one of important molecules for infant growth, it can prevent NTD (neural tube defect), which can cause damage in brain and backbone of infant. Folic acid has low stability even under exposure in room temperature. To improve its stability is required special approach to protect its degradation during formulation and storage. The purpose of this study is to develop solid lipid nanoparticle to encapstulate folic acid. Solid lipid nanoparticle (SLN) of folic acid was prepared by microemulsion method. SLN consisted of palmitic acid as lipid, cremophor RH-40 as surfactan and ethanol as co-surfactan. To obtain good characteristic of microemulsion, we studied the influence of drug : lipid ratio and optimized concentration of surfactan and co-surfactan. SLN formed after the microemulsion was precipitated into water at temperature 5oC. Characterization of SLN included the SLN morphology , size and distribution size. The entrapment efficiency of SLN on folic acid indicating the loading capacity of SLN was also determined. The size and morfology of SLN was measured by Scanning Electron Microscope (SEM). Particle size distribution of SLN was measured by Particle Size Distribution Analyzer. Entrapment efficiency of SLN was measured by High performance liquid cromatography (HPLC). Transparent microemulsion and SLN were formed from 20% cremophor RH-40, 10% etanol, 0.015% folic acid and 2% palmitic acid. Particle size distribution of SLN suspension was in the range of 259-877 nm with entrapment efficiency of SLN was 17.28%. Various factors influenced SLN characteristic including type of lipid and surfactan, ratio of lipid, surfactan and drug in microemulsion, solidification process and solubility of drug in lipid.
Copyrights : Copyright Â(c) 2001 by ITB Central Library. Verbatim copying and distribution of this entire article is permitted by author in any medium, provided this notice is preserved.
| Property | Value |
|---|---|
| Publisher ID | JBPTITBPP |
| Organization | S1 - Department of Pharmacy |
| Contact Name | Drs. Mahmudin, SIP. |
| Address | Jl. Ganesha 10 |
| City | Bandung |
| Region | Jawa Barat |
| Country | Indonesia |
| Phone | 62-22-2509118, 2500089 |
| Fax | 62-22-2500089 |
| Administrator E-mail | digilib@lib.itb.ac.id |
| CKO E-mail | digilib@lib.itb.ac.id |
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Contributor...
- Pembimbing: Dr. Sasanti Tarini Darijanto dan Dr. Heni Rachmawati, Editor: Vika A. Kovariansi
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File : 2008 TA PP DEVI FAJAR SUHENDAR 1-COVER.pdf
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File : 2008 TA PP DEVI FAJAR SUHENDAR 1-BAB 1.pdf
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File : 2008 TA PP DEVI FAJAR SUHENDAR 1-BAB 2.pdf
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File : 2008 TA PP DEVI FAJAR SUHENDAR 1-BAB 3.pdf
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File : 2008 TA PP DEVI FAJAR SUHENDAR 1-BAB 4.pdf
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File : 2008 TA PP DEVI FAJAR SUHENDAR 1-BAB 5.pdf
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File : 2008 TA PP DEVI FAJAR SUHENDAR 1-BAB 6.pdf
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File : 2008 TA PP DEVI FAJAR SUHENDAR 1-PUSTAKA.pdf
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