Path: TopS1-Final ProjectMicrobiology2017

OPTIMASI PRODUKSI ENZIM LAKASE OLEH Marasmius sp. DARI TANDAN AREN (Arenga pinnata) DENGAN TRICKLE-BED REACTOR

Undergraduate Theses from JBPTITBPP / 2017-09-15 13:51:32
Oleh : CYNTHIA GHANIYYU MAGDA (NIM : 10413011), School of Life Sciences and Technology
Dibuat : 2017-09-06, dengan 1 file

Keyword : laju aerasi, lakase, Marasmius sp., tandan aren, trickle-bed reactor

Lakase merupakan enzim lignoselulase yang dimanfaatkan pada industri makanan, kertas, maupun tekstil. Namun, kebutuhan lakase di Indonesia yang semakin meningkat tidak diiringi dengan produksi enzim yang mencukupi. Salah satu substrat yang mengandung lignoselulosa dan belum pernah dimanfaatkan dalam produksi lakase adalah tandan aren. Tandan aren merupakan limbah pengolahan gula aren. Produksi gula aren di Indonesia mencapai 30.376 ton/tahun, sehingga menghasilkan limbah dengan jumlah tinggi. Penelitian ini dilakukan untuk mengetahui produksi optimum enzim lakase oleh Marasmius sp. pada laju aerasi yang berbeda dengan substrat tandan aren. Optimasi produksi lakase oleh Marasmius sp. dilakukan dengan sistem fermentasi substrat padat pada trickle-bed reactor bervolume 1 Liter. Fermentasi dilakukan selama 14 hari menggunakan substrat tandan aren yang dialiri medium kirk dengan kosubstrat berupa glukosa 1g/L. Laju aerasi yang digunakan yaitu 1 vvm, 1,5 vvm, dan 2 vvm. Kondisi awal fermentasi yaitu pada pH 4,5, laju alir medium sebesar 1,5 mL/menit, serta suhu selama fermentasi yaitu 32oC. Analisis yang dilakukan selama produksi enzim meliputi aktivitas enzim lakase, selulase, mangan peroksidase, dan lignin peroksidase, perubahan konsentrasi gula pereduksi, serta perubahan derajat keasaman (pH) yang diukur setiap 12 jam. Hasil analisis menunjukkan perubahan aktivitas lakase bersifat fluktuatif pada seluruh variasi. Aktivitas lakase tertinggi pada aerasi 1 vvm, 1,5 vvm, dan 2 vvm secara berurutan adalah 37,740 U/mL pada hari ke-6 fermentasi, 43,375 U/mL pada hari ke-5 fermentasi, dan 34,726 U/mL pada hari ke-5 fermentasi. Aktivitas enzim selulase, lignin peroksidase, mangan peroksidase tertinggi selama fermentasi secara berurutan adalah 11,051 U/mL, 10,893 U/mL, dan 2,028 U/mL. Derajat keasaman (pH) selama proses fermentasi pada seluruh variasi berada pada rentang 4,50 – 8,46. Konsentrasi gula pereduksi selama proses fermentasi pada seluruh variasi sampel mengalami penurunan pada awal proses fermentasi, kemudian bersifat fluktuatif pada hari ke-3 hingga ke-14 dengan rentang 0,065-0,394 mg/mL. Berdasarkan hasil penelitian tersebut, dapat disimpulkan bahwa laju aerasi terbaik adalah 1,5 vvm dengan aktivitas lakase tertinggi yaitu 43,375 U/mL pada hari ke-5 fermentasi.

Deskripsi Alternatif :

Laccase is a lignocellulase enzyme used in food, paper, and textile industries. However, increasing demand of laccase is not provided by enough enzyme production in Indonesia. A substrate containing lignocellulose which has never been used in laccase production is sugar palm’s bunch. Sugar palm’s bunch is a waste produced in palm sugar processing. Palm sugar production in Indonesia is about 30,376 tons/year, which produces an abundant amount of waste. The aim of the research was to obtain the optimum aeration rate to produce laccase enzyme by Marasmius sp. using sugar palm’s bunch as a substrate. Solid substrate fermentation was used to produce laccase in a litre trickle-bed reactor. Fermentation lasted for 14 days using sugar palm’s bunch as a substrate and trickled down by Kirk’s medium with glucose 1 g/L as cosubstrate. Aeration rate used in the research were 1 vvm, 1,5 vvm and 2 vvm. Initial pH for fermentation was 4,5, medium flow rate was 1,5 mL/min, and fermentation temperature was 32oC. During enzyme production, activity of laccase, cellulase, manganese peroxidase, and lignin peroxidase were assayed. The change in reducer sugar concentration and acidity (pH) were also checked. All of the assays were analyzed every 12 hours. Result showed that change in laccase activity were fluctuating for all of the aeration rate used. The highest laccase activity with aeration rate 1 vvm, 1,5 vvm, and 2 vvm, were 37,740 U/mL on the 6th day of fermentation, 43,375 U/mL on the 5th day of fermentation, dan 34,726 U/mL on the th day of fermentation, respectively. The highest activity of cellulase, lignin peroxidase, and manganese peroxidase were 11,051 U/mL, 10,893 U/mL, and 2,028 U/mL, respectively. Acidity (pH) during fermentation process were fluctuating for all of the aeration rate used, which ranges from 4,50 to 8,46. Reducer sugar concentration decreased in the first few days of fermentation, and then it was fluctuating for the rest of fermentation for all of the aeration rate used, which ranges from 0,065mg/mL to 0,394 mg/mL. Based on the result, the best aeration rate is 1,5 vvm with highest laccase activity 43,375 U/mL on the 5th day of fermentation.

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